ʻO ka hopena pale o ka mea antitumor I o Agkistrodon halys venom i ka hoʻonui ʻana o ka maʻi maʻi maʻi kanaka A549

[Abstract] Pahuhopu: E aʻo i ka hopena o ka Agkistrodon acutus venom tumor suppressor component I (AAVC-I) ma ka hoʻonui ʻana a me ka apoptosis o ka maʻi maʻi maʻi maʻi kanaka A549.Nā Kaʻina Hana: Ua ana ʻia nā helu inhibition o AAVC-I ma nā ʻokoʻa like ʻole ma nā cell A549 no 24h a me 48h e ke ʻano MTT;Ua hoʻohana ʻia ka ʻili HE a me ka Hoechst 33258 fluorescence staining e nānā i ka apoptosis mai ka morphology;Ua ʻike ʻia ka ʻōlelo o ka protein bax e ka immunohistochemistry.Nā hualoaʻa: Ua hōʻike ʻo MTT i hiki iā AAVC-I ke kāohi i ka hoʻonui ʻana o nā cell A549 ma kahi ʻano e hilinaʻi ana i ka manawa a me ka hopena;Ma hope o ka mālama ʻana i ka AAVCI no nā hola 24, ʻike ʻia ka pyknosis nuklea, ka hyperchromatic nuklea a me nā kino apoptotic ma lalo o ka microscope;Ua hōʻike ʻo Immunohistochemistry i ka piʻi ʻana o ka nui o ka optical density me ka piʻi ʻana o ka lāʻau lapaʻau, e hōʻike ana i ka hoʻohālikelike ʻana o ka protein bax i hoʻoponopono ʻia.Ka hopena: ʻO ka mea antitumor I o Agkistrodon acutus venom hiki ke kāohi i ka maʻi maʻi ʻaʻai kanaka A549 a hoʻoulu i ka apoptosis, pili paha i ka hoʻoponopono ʻana o ka ʻōlelo bax.