[Abstract] [Pahuhopu] No ka hoʻomākaukau ʻana i ka antibody IgY e kūʻē i ke kumu maʻamau o nā metalloproteinases mai Agkistrodon acutus venom, a no ke aʻo ʻana i kona hopena i ka hana o ka nahesa [Methods] Ma ka nānā ʻana i nā kaʻina maʻamau o nā metalloproteinases like ʻole mai Agkistrodon acutus venom, ua hoʻolālā mākou. a hoʻohui i nā peptides antigen i mālama nui ʻia ʻo PT1 a me PT2, i pili pono i ka hana enzyme;Hoʻopili ʻia nā moa me nā pūhui conjugated KLH-PT1 a me KLH-PT2 e loaʻa ai nā antibodies IgY.Ua aʻo ʻia ka titer o IgY a me nā ʻano cross-rection me Agkistrodon acutus a me Agkistrodon acutus venom e ELISA a me Western blot;ʻO ka mea hope loa, ua loiloi ʻia ka hana neutralizing o IgY e ka anti-subcutaneous hemorrhage test i nā ʻiole [Results] Ua hōʻike ʻia nā hopena o ELISA a me Western blot e hiki i ka anti-KLH-PT1 a me ka anti-Agkistrodon acutus IgY hiki ke hoʻololi i ka pane me Agkistrodon acutus a me Agkistrodon acutus venom. , a ua ʻoi aku ka ikaika o ka hope ma mua o ka mua;ʻAʻole ʻike ʻia ka anti-KLH-PT2 IgY me ka Agkistrodon acutus venom a me Agkistrodon acutus venom in vitro, akā he ikaika neutralizing toxicity i ka hoʻokolohua anti-bleeding in vivo, a ʻoi aku ka maikaʻi ma mua o nā mea ʻelua [Hoʻoholo] ʻO ka IgY Ua hoʻomākaukau ʻia ka antibody i hiki ke hoʻololi i ke ʻano me nā mea ʻawaʻawa e kahe ana i ke koko ma ka hoʻolālā ʻana i ke kaʻina maʻamau o ka antigen peptide o metalloproteinase, ka mea i hoʻokumu i ke kumu no ka hoʻomākaukau ʻana i nā lāʻau ʻawaʻawa kūikawā, maikaʻi, haʻahaʻa a me ka laulā ākea anti-hemorrhagic snake venom antibody.
Ka manawa hoʻouna: Jan-12-2023