[Abstract] Pahuhopu E hoʻoholo i ka N-terminal 15 amino acid koena o ʻelua mau subunits like o Agacutin.Pono e hoʻokaʻawale ʻia nā ʻāpana ʻelua o ka mole enzyme no ka loaʻa ʻana o ke kaulahao peptide hoʻokahi subunit a hoʻopaʻa ʻia.Nā Hana Ua loaʻa iā Agacutin i hoʻomaʻemaʻe loa ʻia e ke ʻano biochemical.Ua hoʻomākaukau ʻia nā ʻāpana ʻāpana hoʻokahi no ka ʻike a me ka hoʻonohonoho ʻana e ka SDS-PAGE electrophoresis separation, subunit adhesive strip recovery a me PVDF membrane electrotransfer technology.Ua hoʻoholo ka mea hope i nā koena amino acid 15 ma ka N-terminal o ka subunit ma ke ʻano hoʻohaʻahaʻa Edman.Nā hualoaʻa ʻO ke kaʻina o nā ʻāpana liʻiliʻi (16 kDa) ʻo DCSSSGWSSYEEHQYY, a ʻo ke kaʻina o nā ʻāpana liʻiliʻi (15 kDa) ʻo DSSGWSSYEGHEYYV.Manaʻo Manaʻo Ua hōʻike ʻia nā hopena sequencing ʻo Agacutin he snake venom thrombin hou e like me ka enzyme ma ka huli ʻana i ka waihona NCBI.
Ka manawa hoʻouna: Dec-22-2022