ʻO ka hopena hoʻopaʻa ʻana i ka tumo o ka venom tumor inhibitory component I ma ka maʻi maʻi ʻaʻai kino kanaka A549

Pahuhopu: E aʻo i ka hopena o ka ʻāpana hoʻopau ʻana i ka maʻi tumora I (AAVC-I) i ka hoʻonui ʻana a me ka apoptosis o ka maʻi maʻi maʻi maʻi kanaka A549.Nā Hana: Ua hoʻohana ʻia ke ʻano MTT no ka ʻike ʻana i ka 24h a me 48h inhibition rate o A549 cell e AAVC-I ma nā ʻano ʻokoʻa.
Ua hoʻohana ʻia ka ʻili HE a me ka Hoechst33258 fluorescent staining e nānā i ka apoptosis mai ka morphology;Ua hoʻohana ʻia ka Immunohistochemistry e ʻike i nā loli i ka hōʻike protein BAX.Nā hualoaʻa: Ua hōʻike ʻo MTT e hiki iā AAVC-I ke kāohi i ka hoʻonui ʻana o nā pūnaewele A549 i ka manawa- a me ke ʻano i hilinaʻi ʻia.Ma hope o ka mālama ʻana i ka AAVCI no 24h, ʻike ʻia ka nucleus contraction, nuclear hyperstaining a me nā kino apoptotic ma lalo o ka microscopy.Ua hōʻike ʻo Immunohistochemistry i ka piʻi ʻana o ka nui o ka optical density me ka piʻi ʻana o ka hoʻonui ʻana i ka lāʻau, e hōʻike ana ua hoʻonui ʻia ka ʻōlelo protein BAX.